![]() ![]() Turn the plates upside down and put them in a warm place. "Place each Petri dish inside a zip lock bag to prevent drying out and to control odors. Here's some information about making a light bulb incubator for your plates: Be sure to read this information about working with agar if you haven't yet. I am assuming that when you said agar you meant NUTRIENT agar because plain agar by itself would not support growth of microbes. A commonly used prepared medium sold by scientific supply companies such as Carolina Biologicals is called 'nutrient agar' and it contains glucose, yeast extract, a tryptic protein digest and various salts. Nutrients have to be added to agar for bacteria to feed on and grow. ![]() What kind of agar are you using? Technically agar is JUST the support for the growth of microorganisms. If you have a magnifying glass, take a look at the surface of the agar and see if there are any tiny colonies of bacteria or fungi growing. They do take time to form visible colonies, especially at cooler temperatures, so I would give the plates a few more days. Sorry to hear your bacterial cultures are not growing as they should. Any tips or explaining what I'm doing wrong would be greatly appreciated. I have time until my project is due, I just wanted everything done early honestly. I was looking to start working on my board this weekend, but with the bacteria, I just don't see it happening. My two other experiments are going very well, just this one is wrong. Am I doing something wrong? I'm just not sure. Now that it's Thursday, I'm still not seeing any sign of growth. I stored the samples inside a plastic bag and put them upside down in a warm dark place. I collected bacteria from my laptop and door handle (there was plenty of dirt on the swabs from my laptop) and transferred the samples to my agar (without tearing it, i took precaution). When I realized my mistake on Tuesday, I prepared it again (right this time). ![]() I bought agar I had to prepare myself and when I first started my experiments last Saturday, I prepared it wrong. I'm honestly now sure what I'm doing wrong. One of my experiments for my science fair project is to grow bacteria, I'm testing it for something, but first I need them to start growing. ![]()
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